For an updated list of publications press here
Selected publications
Lane^, B.J., Ma^, Y., Yan^, N., Wang, B., Ackermann, K., Karamanos, T.K., Bode, B.E., and Pliotas*, C. (2024) Monitoring the Conformational Ensemble and Lipid Environment of a Mechanosensitive Channel Under Cyclodextrin-Induced Membrane Tension. Structure 32: 1-12 (doi: 10.1016/j.str.2024.02.020)
Haysom, S.F., Machin, J., Whitehouse, J.M., Horne, J.E., Fenn, K., Ma, Y., Hassane El Mkami, Nils Böhringer, Schäberle, T.F., Ranson, N.A., Radford*, S.E., and Pliotas*, C. (2023) Darobactin B Stabilises a Lateral-Closed Conformation of the BAM Complex in E. coli Cells. Angew Chem Intl Ed p.e202218783 (doi: 10.1002/ange.202218783) – Selected inside Cover in Angewandte Chemie International Edition
Wang^ B., Lane^, B. J., Kapsalis, C., Ault, J. R., Sobott, F., El Mkami, H., Calabrese, A. N., Kalli, A. C., and Pliotas*, C. (2022) Pocket delipidation induced by membrane tension or modification leads to a structurally analogous mechanosensitive channel state. Structure 30: 1-15 (doi: 10.1016/j.str.2021.12.004)
Kapsalis, C., Wang, B., El Mkami, H., Pitt, S. J., Schnell, J. R., Smith, T. K., Lippiat, J. D., Bode, B. E., Pliotas*, C. (2019) Allosteric activation of an ion channel triggered by modification of mechanosensitive nanopockets. Nat Commun 10:4619. (doi: 10.1038_s41467-019-12591-x)
Pliotas, C., Dahl, A. C. E., Rasmussen, T., Mahendran, K. R., Smith, T. K., Marius, P., Gault, J., Banda, T., Rasmussen, A., Miller, S., Robinson, C. V., Bayley, H., Sansom, M. S. P., Booth, I. R., and Naismith*, J. H. (2015) The role of lipids in mechanosensation. Nat Struct Mol Biol (doi: 10.1038/nsmb.3120)
All publications
2024
26. Lane^, B.J., Ma^, Y., Yan^, N., Wang, B., Ackermann, K., Karamanos, T.K., Bode, B.E., and Pliotas*, C. (2024) Monitoring the Conformational Ensemble and Lipid Environment of a Mechanosensitive Channel Under Cyclodextrin-Induced Membrane Tension. Structure (accepted)
2023
25. Hardman, K., Goldman, A., and Pliotas*, C. (2023) Membrane force reception: mechanosensation in G-Protein Coupled Receptors and tools to address it. Curr Opin Physiol, 100689 (doi: 10.1016/j.cophys.2023.100689)
24. Haysom, S.F., Machin, J., Whitehouse, J.M., Horne, J.E., Fenn, K., Ma, Y., Hassane El Mkami, Nils Böhringer, Schäberle, T.F., Ranson, N.A., Radford*, S.E., and Pliotas*, C. (2023) Darobactin B Stabilises a Lateral-Closed Conformation of the BAM Complex in E. coli Cells. Angew Chem Intdl Ed, p.e202218783 (doi: 10.1002/ange.202218783)
23. Lane, B. J., and Pliotas*, C. (2023) Approaches for the modulation of mechanosensitive MscL channel pores. Front Chem 11:1162412 (doi.org/10.3389/fchem.2023.1162412)
2022
22. Boakes, J. C., Harborne, S. P. D., Ngo, J. T. S., Pliotas, C., and Goldman*, A. (2022) Novel variants provide differential stabilisation of human equilibrative nucleoside transporter 1 states. Front Mol Biosci 9: 970391 (doi.org/10.3389/fmolb.2022.970391) (Editor selection for the special collection “Understanding Membrane Transporters: From Structure to Function”)
21. Dionysopoulou, M., Yan, N., Wang, B., Pliotas*, C., and Diallinas*, G. (2022) Genetic and cellular characterization of MscS-like putative channels in the filamentous fungus Aspergillus nidulans. Channels 16: 148-158 (https://doi.org/10.1080/19336950.2022.2098661)
20. Lane, B. J., Wang, B., Ma, Y., Calabrese, A. N., El Mkami, H., and Pliotas*, C. (2022) HDX-guided EPR spectroscopy to interrogate membrane protein dynamics. STAR protocols 3:101562 (https://doi.org/10.1016/j.xpro.2022.101562)
19. Wang^ B., Lane^, B. J., Kapsalis, C., Ault, J. R., Sobott, F., El Mkami, H., Calabrese, A. N., Kalli, A. C., and Pliotas*, C. (2022) Pocket delipidation induced by membrane tension or modification leads to a structurally analogous mechanosensitive channel state. Structure 30: 1-15 (https://doi.org/10.1016/j.str.2021.12.004)
2020
18. Hartley^ A. M., Ma^, Y., Lane^ B. J., Wang^ B., Pliotas*, C. (2020) Using pulsed EPR in the structural analysis of integral membrane proteins. Electron Paramag Reson, The Royal Society of Chemistry 27:74 -108.
17. Kapsalis, C., Ma, Y., Bela*, B. E., and Pliotas*, C. (2020) In-Lipid Structure of Pressure-Sensitive Domains Hints Mechanosensitive Channel Functional Diversity. Biophys J 119(2):448-459 (doi: 10.1016/j.bpj.2020.06.012)
16. Koo, C. Z. et al., (2020) The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex. J Biol Chem 295(36):12822-12839 (doi: 10.1074/jbc.RA120.012601)
2019
15. Kapsalis, C., Wang, B., El Mkami, H., Pitt, S. J., Schnell, J. R., Smith, T.K., Lippiat, J.D., Bode, B.E., and Pliotas*, C. (2019) Allosteric activation of an ion channel triggered by modification of mechanosensitive nanopockets. Nat Commun 10:4619. (doi: 10.1038_s41467-019-12591-x)
14. Michou, M, Kapsalis C, Pliotas C., and Skretas* G. (2019) Optimization of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR. ACS Synth Biol 8(7):1631-1641. (doi: 10.1021/acssynbio.9b00120)
2017
13. Ackermann, K., Pliotas, C., Valera, S., Naismith, J.H., and Bode*, B.E. (2017) Sparse labeling PELDOR spectroscopy on multimeric mechanosensitive membrane channels. Biophys J 113 (9): 1968-1978.
12. Pliotas, C et al., (2017) Adenosine Monophosphate Binding Stabilizes the KTN Domain of the Shewanella denitrificans Kef Potassium Efflux System. Biochemistry (doi: 10.1021/acs.biochem.7b00300)
11. Pliotas*, C. (2017) Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR. Methods Enzymol 594:203-242 (doi: 10.1016/bs.mie.2017.05.013)
10. Asadi, J., Ferguson, S., Raja, H., Hacker, C., Marius, P., Ward, R., Pliotas, C., Naismith, J.H., and Lucocq*, J.M. (2017) Enhanced imaging of lipid rich nanoparticles embedded in methylcellulose films for transmission electron microscopy using mixtures of heavy metals. Micron (doi: 10.1016/j.micron.2017.03.019)
9. Pliotas*, C., and Naismith*, J. H. (2017) Spectator no more, the role of the membrane in regulating ion channel function. Curr Opin Struct Biol (doi: 10.1016/j.sbi.2016.10.017)
2016
8. Hacker, C., Asadi, J., Pliotas, C., Ferguson, S., Sherry, L., Marius, P., Tello, J., Jackson, D., Naismith, J.H., and Lucocq*, J.M. (2016) Nanoparticle suspensions enclosed in methyl cellulose: a new approach for quantifying nanoparticles in transmission electron microscopy. Sci Rep (doi: 10.1038/srep25275)
7. Valera, S., Ackermann, K., Pliotas, C., Huang, H., Naismith, J. H., and Bode*, B. E. (2016) Accurate extraction of nanometer distances in multimers by pulse EPR. Chemistry (doi: 10.1002/chem.201505143)
2015
6. Pliotas, C., Dahl, A. C. E., Rasmussen, T., Mahendran, K. R., Smith, T. K., Marius, P., Gault, J., Banda, T., Rasmussen, A., Miller, S., Robinson, C. V., Bayley, H., Sansom, M. S. P., Booth, I. R., and Naismith*, J. H. (2015) The role of lipids in mechanosensation. Nat Struct Mol Biol (doi: 10.1038/nsmb.3120)
2014
5. Ward^, R., Pliotas^, C., Branigan^, E., Hacker, C., Rasmussen, A., Hagelueken, G., Booth, I. R., Miller, S., Lucocq, J., Naismith*, J. H., and Schiemann*, O. (2014) Probing the structure of the mechanosensitive channel of small conductance in lipid bilayers with pulsed electron-electron double resonance. Biophys J 106,834-842 (^: joint first author)
4. Healy, J., Ekkerman, S., Pliotas, C., Richard, M., Bartlett, W., Grayer, S.C., Morris, G.M., Miller, S., Booth, I.R., Conway, S.J., and Rasmussen, T. (2014) Understanding the structural requirements for activators of the Kef bacterial potassium efflux system. Biochemistry 53, 1982-1992
2013
3. Branigan^, E., Pliotas^, C., Hagelueken, G., and Naismith*, J.H. (2013) Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding. Nat Protoc 8, 2090-2097 (^: joint first author)
2012
2. Pliotas, C., Ward, R., Branigan, E., Rasmussen, A., Hagelueken, G., Huang, H., Black, S.S., Booth*, I.R., Schiemann*, O., and Naismith*, J.H. (2012) Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy. Proc Natl Acad Sci U S A 109, E2675-2682
2010
1. Roosild*, T. P., Castronovo, S., Healy, J., Miller, S., Pliotas, C., Rasmussen, T., Bartlett, W., Conway, S.J., and Booth*, I.R. (2010) Mechanism of ligand-gated potassium efflux in bacterial pathogens. Proc Natl Acad Sci U S A107, 19784-19789